Single Molecule Biophysics

• Why single molecule?
  • Bulk measurements can only determine averages.
  • Single molecule measurements resolve distinct sub-populations.

• Useful techniques for studying molecules

  • Optical Tweezers

  • FRET (Fluorescence Resonance Energy Transfer)

  • Polarization Anisotropy

• Encapsulating single molecules within aqueous nanodroplets (hydrosomes)

• Why are hydrosomes an interesting way to study single molecules?